SHARP-ID – Shape and Rigidity Profiling via Density-Induced Banding of Red Blood Cells

About the project

About the Project SHARP-ID – Shape and Rigidity Profiling via Density-Induced Banding of Red Blood Cells Research Project Centrifugation of Red Blood Cells (RBCs) in continuous density gradient has recently been suggested for detecting pathological alterations [1]. Interestingly, band patterns form along the gradient. This project builds on recent findings showing these patterns result from a balance between buoyancy and aggregation forces [2]. It aims to optimize detection of RBCs with altered mechanical properties by refining non-linear density gradients. The goal is to improve reproducibility and sensitivity of band formation to detect mechanical changes: linear gradients are sensitive but poorly reproducible; sigmoidal gradients are stable but lack sensitivity. We aim to design custom gradients combining both advantages. Programmable syringe pumps will generate arbitrary gradients from two density solutions. These will be tested on RBCs with controlled shape and rigidity variations, and quantified using confocal and atomic force microscopy. Band patterns will be analyzed via automated image processing and classified using AI trained on simulated and experimental data. This approach offers a scalable, low-cost diagnostic tool for diseases like sickle cell anemia and malaria, with potential for treatment monitoring and early detection. This project benefits from the interdisciplinary environment at UoB, drawing on expertise from Prof. Ash Toye and Dr. Jan Frayne in red blood cell biology. Clinical insight will be provided by Dr. Claire Rice and Dr. Sanne Lugthart, specialists in inherited blood disorders. The theoretical components will be supported through collaboration with Francesco Turci and Simon Hanna, and access to the Isambard-AI supercomputer. Overall, this environment offers scientific excellence and strong pastoral support within the vibrant research culture of the School of Physics and the EngBioCDT programme. A world-class environment for your research degree The School of Physics is offering a portfolio of PhD Scholarships across all its world-class research groups. These prestigious Scholarships represent a great opportunity to start your Postgraduate Research Career working with world-class academics in a supportive training and development environment. Physics at Bristol was ranked 5th for Research in the 2022 Research Excellence Framework (REF) results, and the University of Bristol is truly world-leading: 55th in the QS World University Ranking (2024) and 81st in the Times Higher Ranking (2024). What does a Scholarship cover? For home students, the scholarships will cover living expenses (UKRI rate), University tuition fees (see PhD Physics | Study at Bristol | University of Bristol ), and training and support expenses. We also welcome applications from outstanding international candidates: we are able to offer a very small number of fully-funded places, but also accept students who can provide full or partial funding from other sources. If you need an offer letter to apply for a competitive scholarship, please get in contact with us well before any deadlines. The Bristol Physics Graduate Research Community If successful, you will become a member of the Physics Graduate School, a vibrant and diverse community of 200-plus research students from a wide range of countries and backgrounds. The Graduate School: runs a comprehensive induction programme to get you off to the best possible start in your research, and a rolling programme of targeted skills training on writing, presenting, project-planning, etc. operates a “one-stop shop” approach to help you navigate through the University procedures organises meetings for postgraduate opportunities, and support in transitioning to post-PhD careers connects you to the wider University, including the Bristol Doctoral College, www.bristol.ac.uk/doctoral-college/ . How do I apply? You will need (1) a CV, (2) a Personal Statement, which is a one- to two-page document introducing yourself and outlining your motivation for PhD research, and (3) a transcript of any qualifying degrees (completed and/or underway). You do not need a research proposal but you must specify a research theme or academic supervisor. Please contact the academics within the School of Physics research areas for information. Applicants will be assessed on merit, and interviews will be held for shortlisted applicants. You need to apply through the University of Bristol application system at http://www.bristol.ac.uk/study/postgraduate/apply/ . Please select Physics PhD on the Programme Choice page. The School of Physics supports Diversity and Equality, and we invite all eligible candidates to apply. We encourage applications from under-represented groups. We hold a Bronze Athena Swan award and a Physics Juno Champion award. Please specify “Bristol Physics Research Scholarships ” on the application form as your source of potential funding. Early application is encouraged. The closing date for applications to this call is 19 January 2026. What are the candidate requirements? A first degree in physics or a related subject, normally at a level equivalent to at least UK upper second-class honours, or a relevant postgraduate master's qualification. Please see Applying from your country | International students | University of Bristol on the International Office website. English language requirements If English is not your first language, you need to meet English Language Profile F. Further information about this can be found here: English language requirements | Study at Bristol | University of Bristol Admissions Statement: PhD and MSc by Research Physics Admissions Statement Contacts and further information Please contact the Bristol Physics Graduate School at: physics-pg@bristol.ac.uk Direct inquiries to the project supervisor are also welcome at alexis.darras@bristol.ac.uk

Research areas